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The obtained active site model was further substantiated as amino acid residues Arg 166 , Arg 284 and Ser 447 were located at an appropriate distance for hydrogen bonding interaction with the carboxylate group of the substrate

Since the increase in absorbency is directly proportional to the amount of captured GLP-1 Total in the unknown sample, the latter can be derived by interpolation from a reference curve generated in the same assay with reference standards of known concentrations of GLP-1.

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