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Even then, it is not harmful, though it may lose some efficacy

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Regulation of Active Site Coupling in Glutaminedependent NAD(+) Synthetase of the Article. Nature Structural & Molecular Biology 16(4): 421429

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The well content was quickly detached using a cell scraper and collected in microcentrifuge tube (Sarstedt), directly snap-frozen in liquid nitrogen, and stored at 80 C until further use

DEX significantly altered the expression of key regulatory genes, while Spirulina co-treatment modulated these alterations in a dose-dependent manner, suggesting its potential as a therapeutic nutraceutical