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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM

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Investigating potential contributors such as microvascular injury, inflammatory processes, alterations in nerve metabolism, and the role of specific glycemic control strategies is crucial for unraveling the etiology of TIND

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Patients with PD had thinner average RNFLs, indicating potential diagnostic significance of RNFL thickness (Alves et al., 2023) (see Table 7)