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(DOI 10.54499/2021.05487.BD), and ITR Laboratory for Integrative and Translational Research in Population Health (LA/P/0064/2020, DOI 10.54499/LA/P/0064/2020)

Remove any air bubbles While the needle is still in the vial, tap the syringe to push any bubbles to the top and gently depress the plunger to push the air bubbles out

Pull 1 milliliter (or cubic centimeters abbreviated cc on a syringe) of water from the vial into a syringe and then squirt it into the vial containing the powder

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