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Researchers first depleted cellular glutathione using buthionine sulfoximine (BSO) , then measured restoration rates using either 10 mM LiposoMax Liposomal Glutathione or 10 mM standard glutathione

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For NADP + and NADPH assays, 20 L (for NADP + assay) or 30 L (for NADPH assay) of the neutralized sample extract was combined with 90 L of assay mix consisting of 50 L of 0.1 M HEPES containing 2 mM EDTA (pH 7.5), 20 L of 1.2 mM 2,6-dichlorophenolindophenol, 10 L of 20 mM phenazine methosulfate, and 10 L of 10 mM glucose-6-phosphate