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Washing was repeated once before cells were gently resuspended and rested overnight in 1 mL of pre-warmed RPMI 1640 media (Gibco) supplemented with 10% FBS (Biological Industries), L-glutamine (2 mM), penicillin (100 U/mL, Biological Industries) and streptomycin (0.1 mg/mL, Biological Industries), in an incubator at 37 C and 5% CO 2

(2021) Long-term metformin therapy and vitamin B12 deficiency: An association to bear in mind

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