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SDS-PAGE electrophoresis and Western blot The formation of AX-serum proteins adducts during the RAST inhibition assay, as a potential source of carriers, was assessed by collecting the sera samples after 3 h of incubation with AX (1, 10 and 100 mM)

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hCG was also shown to upregulate the expression of IGFR in rat Leydig cells suggesting cross-talk between both pathways (90, 103)

The trial showed the ability to discontinue insulin use and resulted in improved consistency in blood glucose levels, minimized hypoglycemia and hyperglycemia episodes, and a decrease in HbA1c level

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