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Although not assessed here, a coordinated increase in superoxide dismutase activity could have elevated H 2 O 2 production in the 500 M EGT-treated broccoli ( Figure 3 )

Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)