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Trypsin (TPCK-treated, from bovine pancreas), pepsin (from porcine gastric mucosa), dithiothreitol (DTT), acetaminophen (APAP), HPLC-grade acetonitrile (ACN) and methanol (MeOH), iodoacetamide (IAM), glucose-6-phosphate dehydrogenase (type XV, from baker's yeast), and other chemicals were purchased from Sigma-Aldrich (St

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Start at lower doses to assess tolerance and adjust based on results