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As early as the 1980s, clinical studies reported that obese individuals had circadian prolactin secretion rhythm disorders linked to adverse metabolic phenotypes, including insulin resistance, dyslipidemia, and systemic inflammatory responses

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Transferrin: to downregulate TfR1, cells were incubated overnight with 100 M human holo-Tf (Sigma-Aldrich, United States) in endotoxin-free PBS (EMD Millipore) based on published studies