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We directly confirmed that the products of the Cr GPDH catalyzed reaction are glycerol and Pi, and that this reaction is not detected in the presence of NADPH or the absence of MgCl 2 , as Mg 2+ is the cofactor for the phosphatase reaction ( Figure 2b-d )

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The membrane was visualized using the chemiluminescence system

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Available online at: 45 LiW.ZhouX.XuS.CaoN.LiB.ChenW.et al (2022)