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Secondary antibodies included Secondary donkey IgG directed against goat IgG conjugated with Cy5(Jackson ImmunoResearch Labs Cat# 705-175-147, RRID: AB_2340415), Secondary donkey IgG directed against rabbit IgG conjugated with Cy3(Jackson ImmunoResearch Labs Cat# 711-165-152, RRID: AB_2307443) and Secondary donkey IgG directed against mouse IgG conjugated with 488(Jackson ImmunoResearch Labs Cat# 715-545-150, RRID: AB_2340846), both at a dilution of 1:200 (Molecular Probes, Eugene, OR, USA)
Phosphorylation occurs on various cellular substrates on tyrosine residues including the IRS family and Src homology/collagen (SHC)-transforming family of proteins that mediate signals from the tyrosine kinases [132]
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The most common AEs of SGLT2is are genital mycotic infections [4, 11]
MATERIALS AND METHODS: We measured the expression of ghrelin and growth hormone secretagogue receptor 1a (GHS-R1a) in rat osteoblasts using RT-PCR and immunohistochemistry (IHC)
