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To describe and analyse metabolism in general and metabolic alterations specifically at a genome-scale level, metabolic network modelling using constraint-based approaches has been successfully applied for various cancer [19, 20] and many other diseases, e.g

A consequence of hepatocyte necrosis is the release of a series of cellular constituents, such as high mobility group box-1 (HMGB1) protein (92), ATP (93), mitochondrial DNA (94) and nuclear DNA fragments (91)

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