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Western blotting Total protein was extracted from paraformaldehyde-fixed, paraffin-embedded pancreatic tissues using the FFPE Total Protein Extraction Kit (Sangon Biotech, Shanghai, China) or from cultured PSCs using RIPA Lysis Buffer (Beyotime)

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Effects of boiling on folate retention were evaluated in soaked mung beans, adzuki beans, cowpeas, faba beans, peas, and common beans