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Circadian regulation of glutathione levels and biosynthesis in Drosophila melanogaster

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The overwhelming dominance of ROS generated by enzymes like NADPH oxidases and xanthine oxidase over antioxidants like superoxide dismutase causes cell injury and tissue damage through direct injury, lipid peroxidation and protein oxidation leading to protease release and antioxidant and antiprotease enzyme inactivation as well as alteration of transcription factors activator protein-1 and NFB

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Neurons were visualized with infrared differential interference contrast imaging and a CCD camera under a water-immersion objective (40 , 0.8 numerical aperture)

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