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Tissue was homogenized in TRI Reagent , mixed with ethanol, and loaded onto a Zymo-Spin IIICG Column, followed by centrifugation

Large portion sizes and overeating should be avoided

When patients are trying to increase protein intake, it doesnt need to be complicated

TriNetX platform study specifically excluded patients with prior acetazolamide use at baseline to capture new prescriptions during follow-up