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The unsupervised clustering of 4453 fibroblasts was used to analyze the senescent cells in keloid and normal skin fibroblast based on senescence-associated marker genes, CDKN2A, TP53, CDKN1A (also known as p21 ) , LMNB1, H2AFX, TP53BP1, SFRP4, CCND1 and GLB1 according as previously reference 20,21

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Cells were transfected with corresponding expression constructs for 24 h before 4% formaldehyde fixation

This is possibly due to the formation of protein mixed disulphides (44), or reduction in activity of glutathione reductase, such as that suggested from studies of squirrel lenses (194)

doi: 10.1016/0006-2952(96)00241-9