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A data-independent (DIA) acquisition method was used, in which one full scan (3001,650 m/z , maximum ion fill time of 45 ms, normalized AGC target 300%, R = 120.000 at 200 m/z ) was followed by 66 tMS2 fragment scans of unequally spaced windows with 1 Th overlap, covering the same m/z range (fill time 22 ms, normalized AGC target 1,000%, normalized high-collision density energy of 30%, R = 15.000)

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