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Glutathione acts as a shield for the mitochondria, ensuring they can produce energy efficiently without being damaged by their own metabolic byproducts

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The half-lives of unbound IGF-1, IGF-1 in a binary complex, and IGF-1 in a ternary complex are less than 10 minutes, 25 minutes, and more than 16 hours respectively meaning that IGF-1 activity is tightly regulated by its binding proteins

Off-target detection To determine the site-specific cleavage of the CRISPRCas9 system in vivo, the potential off-target loci were searched using Cas-OFFinder ( The selected potential off-target sites were initially PCR-amplified and then subjected to a DNA sequencing assay

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