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Read on to learn more about what a diabetic ulcer is, what its symptoms are, how to treat it, and how it can be prevented

To prepare the single-cell suspension, purified islets were digested with 0.25% trypsin-EDTA and filtered through a 40 m cell strainer

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Best practice is to reconstitute all vials identically (same diluent volume) so injection volume remains constant throughout the protocol

Dose reduction of concomitant glucose-lowering medications may be required, and patients should be educated about hypoglycaemia recognition and management

Dulbecco's modified Eagle's medium for cell culture, fetal bovine serum, and trypsin were purchased from Gibco (Grand Island, USA)