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The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide

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Stepniak E, Ricci R, Eferl R, Sumara G, Sumara I, Rath M, Hui L et al

[DOI] [PMC free article] [PubMed] [Google Scholar] 102.Saadat M

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If it delivers on the promise suggested by preclinical research, it would represent a meaningful addition to GERD treatment options