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Methods Insect cell expression HA-signal peptide-FLAG-3C-GLP-1R-3C-8HIS 13 , human DNG s 46 , His 6 -tagged human G 1 and G 2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described

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As ultimately the precursor must originate from the TCA cycle in the form of an intermediary which most likely is -ketoglutarate (Figure 5) an anaplerotic mechanism must be operating involving de novo synthesis of oxaloacetate