P., Sablonniere, B., Guieu, R., et al
SSRIs, SNRIs, MAOIs, TCAs are hard contraindications even at low topical concentrations.
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Staying well-hydrated will make the process smoother and more comfortable for you
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Furthermore, inclusion of a His-tag during expression (for subsequent Ni-nitrilotriacetic acid purification) might alter protein folding and thus binding results (Ledent et al., Table 2)