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Mahmoud HS, Abou-El-Naga M, Omar NAA, et al

Another clinical study of 71 women with mild to advanced signs of photoaging who applied GHK-Cu facial cream daily for 12 weeks showed increased skin density and thickness while reducing sagging and the appearance of fine lines and wrinkles

Notably, endogenous GHK-Cu levels in human plasma are age-dependent, dropping from ~200 ng/mL at age 20 to below 80 ng/mL after age 60

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Protein concentration of the cell extracts was determined by Bradford assay (Bio-Rad Laboratories, CA, USA)

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