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Sanitise both rubber stoppers with an alcohol swab

Special features Free of fragrance, oils, silicones, sulfates, harsh alcohols & parabens

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In accordance with ethicalguidelines, approval was waived by the Research Ethics Committee at King Abdulaziz University

Eur Bull Drug Res

For fixed imaging, worms were first washed three times with 1 mL of 0.1% Tween in 1 PBS, resuspended in 1 mL of 100% methanol, and incubated at 30 C for 5 min