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The LEAN trial (Liraglutide Efficacy and Action in NASH) was a landmark study published in The Lancet (2016) demonstrating that liraglutide 1.8 mg daily led to NASH resolution in 39% of patients compared to 9% in the placebo group after 48 weeks

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Following centrifugation at 300 g for 5 min, the cell pellets were resuspended in phosphate buffered saline (PBS) and then analyzed with a NovoCyte flow cytometer (Agilent, CA, USA)