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Overall, 13 portal tracts were analyzed from each liver, and a total of 10 tracts from NAC treated mice, and 8 tracts from untreated mice were analyzed

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The liver then incorporates some of the L-alpha-alanine into proteins, and the leftover L-alpha-alanine serves as a precursor for the production of urea, which is then excreted in urine

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After beads wash, a single band of GST-X was detected on the beads, indicating high purifty of this sample