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The remaining supernatant was mixed with 10 mM N-ethylmaleimide, rotated at 4 C for 1 h and precleared with Pierce Protein G magnetic beads (Thermo Fisher Scientific, 88848) by rotating at 4 C for 1 h

(d) The 6,7-dihydroxy groups on ring A (Figure 7)

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Repigmentation of vitiligo using the follicular unit extraction technique

After 24 h, cells were expanded into six-well dishes and selected in 1 g ml 1 puromycin for 3 days, then further expanded in nonselective medium