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They might forget main details, big ideas, or whole talks that happened not long ago

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After three cycles of freezing and thawing, the cultures were centrifuged at 1000 g for 20 min (4C) to remove cellular debris, and clarified supernatants stored at -80C

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In neurons, excess intracellular zinc impairs mitochondrial function by dissipating the membrane potential, inhibiting ATP production, increasing ROS generation and permeability transition, disrupting calcium homeostasis and altering mitochondrial dynamics, contributing to neurodegenerative processes 153,154