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Furthermore, the elderly population shows heightened vulnerability to anticholinergic-induced cognitive decline, correlating with widespread use of such medications for urinary incontinence, allergies, and depression [46, 135]

The beads were then washed three times with 1 ml of RIPA buffer, followed by washes with 1 M KCl, 0.1 M Na 2 CO 2 and 2 M urea in 10 mM Tris-HCl (pH 8.0)

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Because melasma involves multiple triggers, it is often considered a complex pigment disorder rather than a simple discoloration