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Equal amounts of protein were loaded and resolved on 10% SDS polyacrylamide gels followed by transfer onto PVDF membranes, which were then blocked with 5% non-fat milk in Tris-buffered saline and 0.1% tween-20 (TBST) for 1 h at room temperature and then incubated with their corresponding primary antibodies, METTL3(1:1000, TA811770, OriGene), METTL14(1:1000, TA378477, OriGene), PDX1(1:1000, TA321081, OriGene), and -actin(1:2000, TA811000, OriGene), overnight at 4 C
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However, the incidence may vary among individuals
