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Real-time PCR was performed using an S1000 PCR instrument (Bio-Rad, Hercules, CA, USA) to quantify gene expression

Specifically, 8 of the 11 sites are fully identical over the 10 residues spanning the P5 to P5 positions, and only two of the 11 cleavage sites have more than a single amino acid difference within the 10 residue span (Figure S1)

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Given the large population affected by gallbladder stones, finding efficient non-surgical treatment methods makes sense

Thymosin Beta 4 Biochemically, Thymosin Beta 4 is a small peptide, i.e., it is composed of the chemically bonded amino acids via the peptide linkages and having amino group at one terminal while having carboxyl group on the other terminal