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What a COA (Certificate of Analysis) Should Include Identity Confirmation Mass spectrometry confirming the correct molecular weight (4,113.58 Da for semaglutide) Amino acid sequence verification with Aib-8 and Arg-34 modifications Confirmation of the C18 fatty-diacid side chain conjugation on lysine-26 Purity Testing HPLC purity look for 98%+ purity The chromatogram should show a single dominant peak with minimal des-acyl or truncated impurities Contamination Testing Endotoxin levels (bacterial contamination) Residual solvent analysis (acetonitrile, TFA) Heavy metals screening How to Verify a COA Is Legitimate Check the testing lab Reputable labs include Janoshik Analytical, MZ Biolabs, and Colmaric Analyticals
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