Melvin et al
Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
Some providers split the dose into two smaller injections per day (morning and evening) for sustained signaling, though once daily is most common
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Because the half-life is 20-30 hours, one injection per day produces relatively stable receptor coverage
