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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

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A quantity of 8.4 g of either one or a mix of the shRNA vectors (sh1-sh4 or shSCR), or a control plasmid expressing green fluorescent protein (GFP) only, were transfected with 10 g of BDNF expression vector into the 293T cells using the calcium phosphate method

Intestinal Cyp24a1 regulates vitamin D locally independent of systemic regulation by renal Cyp24a1 in mice

Bile acids: chemistry, pathochemistry, biology, pathobiology, and therapeutics