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While some practitioners suggest injecting near target areas based on regional absorption theory, scientific evidence for site-specific enhancement is limited

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Philp and colleagues (FASEB J, 2003) showed the LKKTETQ actin-binding site alone is sufficient to promote endothelial cell migration and angiogenesis, and Bock-Marquette and colleagues (Nature, 2004) reported activation of integrin-linked kinase and Akt with cardiomyocyte and epicardial cell migration in mouse cardiac injury models, indicating a signalling role separate from actin buffering