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Subsequently, structural modelling and refinement were performed using COOT and REFMAC5 33,34

Smooth muscle relaxation occurs through a decrease in [Ca 2+ ] c (Somlyo and Somlyo, 2003), either via an electromechanical mechanismcharacterized by membrane re-polarization or hyperpolarizationor through a pharmacomechanical mechanism, which involves membrane receptor activation and inhibition of the intracellular signaling pathways that drive contraction (Woodrum and Brophy, 2001) (Figure 6)

PMID 8598105.

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Moreover, biomarkers related to inflammation, mitochondrial health, and oxidative stress indicated biological improvements during SFN therapy