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After 28 days, the solution should be discarded and a fresh vial reconstituted if ongoing research requires it

The collected slides were stained with Hematoxylin and Eosin (H&E) for histological inspection using a LEICA (DFC290 HD system digital camera, Heerbrugg, Switzerland) attached to a light microscope with 10, 20, and 40 objective lenses 49