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The purified TRF1 TRFH domain was then concentrated by centrifugation on a Vivaspin Turbo 15 centrifugal concentrator (10 kDa MWCO) to either ~ 3 mg/mL for NMR studies or ~ 30 mg/mL for crystallisation before flash-freezing and storage at 80 C (Supplementary Fig

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Spicer, J., Malaspina, D., Blank, S

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53, 54 One study of mitochondrial dysfunction in the autism brain found decreased protein levels of multiple ETC complexes in the cerebellum, frontal and temporal cortex