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Despite their frequent occurrence, the underlying molecular mechanisms contributing to the pathogenesis of varicose veins remain incompletely understood, with oxidative stress and inflammation playing crucial roles in the pathophysiology of disease (recently reviewed in Refs

The cuvettes containing 1.5 mL of 100 mM Tris HCI buffer (pH 8.2), 0.5 mL of 6 mM EDTA, 1 mL of 6 mM pyrogallol solution and 0.1 mL of enzyme extract were put in a spectrophotometer

Frontin-Rubio et al