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After washing with PBS (3 5 min), sections were incubated with species-matched Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen) for 2 h at RT protected from light

Glutathione is water-soluble, and your joints require hydration to maintain the "cushion" that collagen helps build

The back of the neck is a better area than high-friction zones like the palms and feet

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Take a small amount of moisturiser, warm it between your fingers and massage it into your face and neck

The percentage that reaches systemic circulation intact is estimated to be quite low, though exact figures for GHK-Cu specifically aren't well established in published literature