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Klein JA, Ackerman SL (2003) Oxidative stress, cell cycle, and neurodegeneration

[PMC free article: PMC9542252] [PubMed: 35441470] 38

Notable for: Multi-database design (improves generalizability) Active comparator framework Emphasis on short-term safety with acknowledgment of need for longer follow-up Diabetes Care Retrospective Cohort Evaluated thyroid tumors (broader outcome than confirmed malignancies) and found no increased risk with GLP-1 RA exposure in type 2 diabetes patients initiating second-line treatments

Only very small molecules can reliably pass through the skin, which is why patches work for things like nicotine, certain hormones (like estrogen), or some pain medications

Cells were maintained in a humidified environment of 5% CO 2 at 37 C, cultured until an optical confluence of 75-100%, detached with 0.05% trypsin-EDTA 1 (25300-054, Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, USA), at 37 C for approximately 5 min, and split to new plates according to the experimental procedures

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