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Subsequently, 1 g of RNA was reverse-transcribed into cDNA using a commercial kit (Cat# R433-01, Vazyme Biotech Co., Ltd., China), and the resulting cDNA was subjected to quantitative real-time PCR (SYBR, Cat# AG11701, Accurate biotechnology (Hunan) Co., Ltd., Changsha, China)

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Doctors may also give words of encouragement to help patients relax