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however, because of the higher presence of intracellular proteins (cytoskeletal proteins in the T cell and CAR-T cell cocultures and mitochondrial proteins in the TCR-T cell cocultures), we applied a less stringent cutoff (10% instead of 5% FDR) (Supplementary Fig

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4) Centrifuge the lysate at 12,000 rpm for 15-25 minutes at 4C to pellet the cellular debris