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Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency

It said that Vitamin B12 deficiency is common among older adults and can have significant health implications if left untreated. Patients with Neurological Manifestations Those with neurological symptoms from B12 deficiency need special care

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