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Reads containing an IS that matched the Tn5 transposon vector in the donor strain were also discarded

Furthermore, 16S rRNA sequencing revealed that CB 0313.1 upregulated the Firmicutes/Bacteroidetes ratio, enriched Clostridium , and promoted butyrate-producing taxa

Moreover, WRN deficiency resulted in decreased mitochondrial NAD + and altered expression of key salvage pathway enzymes, including NMNAT1 and NAMPT (Lautrup et al