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Cells were subsequently washed twice in PBS and incubated in blocking buffer (20% FCS, 1% Tween-20 in PBS) for 1 h

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284 , F840F851 (2003)

2.2 Environmental factors Multiple environmental exposures contribute to the onset and progression of UC by altering gut microbiota composition, compromising epithelial barrier integrity, and modulating mucosal immunity

Therefore, proper medical assessment is essential before attributing nerve pain solely to vitamin B12 levels

Sometimes ileostomy is also done at this time, which is a procedure that brings the ileum, or end of the small intestine, through an opening in the abdominal wall to allow intestinal waste to drain out of the body