Even if changes occur, they dont necessarily reverse at the same speed as tanning effects
We only used a fork to stir the mix in so we could see how it would respond to a poor mixing technique
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While freezing did not impact the concentrations of NAD + , NADH, GSH, or GSSG, it triggered the conversion of NADPH to NADP + , with about 508% of NADP + originating from NADPH oxidation (Supplementary Figure 1m-r)
Common in-vitro readouts include scratch-wound migration assays and angiogenesis tube-formation assays, alongside investigations of fibroblast behavior and nitric-oxide and growth-factor pathways
10.1016/j.redox.2017.09.002 Redox Biol