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The flow rate was set as 0.3 mL/min

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An aliquot of 10 L of 5 mg/mL MTT solution was added to each well and incubated in the cell culture incubator for 4 h

They are classified as "essential" or "non-essential", with essential amino acids meaning they cannot be manufactured by the body and must be supplied in the diet to maintain good all-round health