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For the immunofluorescence reaction, sections were rinsed in KPBS and incubated for 90 min in AlexaFluor 488 -conjugated secondary antibody (1:500, Jackson Laboratories)

At the same time, the researchers also confirmed that TMAO can accelerate the formation process of NLRP3 inflammasome in kidney tissue and the activation of lytic caspase-1 and IL-1, once again proving that the results of Kapetanaki et al

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Use sunscreen regularly to help prevent worsening of pigmentation